I heard Philip Selenko give a great talk a couple of years back  and have since been (very slowly) getting acquainted with the literature on post-translational modifications (PTMs) in proteins. Last year I wrote about the role of PTMs in protein folding and how we are now starting to look at disordered regions in proteins differently [ ]. PTMs also occur in highly structured regions of the protein. There are over 400 different types of PTMs  , each with the potential to drastically change the conformational space of the protein and hence its function. The high diversity of PTMs and their reversible nature make them a crucial part of understanding protein function, signaling pathways, allostery, binding and even protein energy landscapes.
Among the hundreds of different types of PTMs, phosphorylation (On serine, threonine and tyrosine) is one of the most well studied (mass spectrometry(MS)-based proteomics is now a pretty large field  ). Although a complete list of phosphosites is not yet available, the central question remains how to link the known PTM sites to conformational changes and therefore function. Conservation of PTM is one way to identify functionally relevant sites (this is not to say all functionally relevant sites are conserved) and hence understand protein regulation and their role in protein interaction network. For example, kinases have preferences for certain specific residues near the target phosphorylated site, and identifying the conservation of such sequences can be used to predict regulated sites  .
- Only 39.8% of phosphosites were found to be conserved in one or more species. (The data on identified phosphosites is largely incomplete and hence must be kept in mind while interpreting all data related PTM studies. )
- The fraction of sites with known function increased with the level of conservation across species, thus suggesting that conserved sites are more likely to have function.
- For example, a phosphosite in the activation loop of is one of the more conserved sites across species. Similarly, the conserved site in NDP Kinase A is located near the active site.
Figure 1: Example comparative models with highly conserved phosphorylation sites. The phosphorylation site is highlighted in red. For the NDP kinase A, the structure represents the homo-oligomeric complex. One of the subunits is indicated in blue, with the phosphosite position in red and the substrate in the ball-and-stick representation. [from footnote 6]
- About 20% of the phosphosites identified appeared to be less solvent exposed, although intuitively, adding a phosphate should make the protein more exposed. The authors suggest that conformational flexibility might play a role, as it is well known that PTMs can change function by altering the conformational space of the protein. If that is the case, then we can use structural information to identify PTMs that can regulate protein conformation. However, if conformational flexibility is playing a role, these regions could also be poorly modeled. As an MD-person, I really think it is a good idea to integrate these structural results with multiple MD studies to get a more complete understanding.